Smear preparation is done to prepare the organism for staining. Smear preparations are used to determine cell shape, arrangement of the cells, and internal structures. You will be preparing a smear for the Gram-stain procedure. The purpose of making a smear is to fix the bacteria onto the slide.
Regarding this, what is the purpose of fixation in smear preparation?
SMEAR PREPARATION. The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
Similarly, how is smear prepared? Preparation of the smear is as follows: From broth: Using a cooled, sterile loop, place a loopful of broth on the slide and spread in a circular motion to about 1 cm in diameter. From plated media: Place a drop of sterile water or saline on the slide. Select the isolated colony to be stained.
Similarly one may ask, what is the purpose of a simple stain?
The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple stain is a very simple staining procedure involving only one stain. You may choose from methylene blue, Gram safranin, and Gram crystal violet.
What is bacterial smear?
A bacterial smear is a thin layer of bacteria placed on a slide for staining.